The DATEDIF function is used in Microsoft Excel to calculate the difference between two dates in various units, such as days, months, or years. The syntax for the function is DATEDIF(startdate, enddate, unit). To calculate the total number of days between two dates, the unit argument is specified as "d". While a simple subtraction formula (e.g., =enddate-startdate) also yields the number of days, DATEDIF is the correct function among the choices provided for this specific calculation. This function is maintained for compatibility with older spreadsheet programs like Lotus 1-2-3.